Composite

Part:BBa_K3127998:Design

Designed by: Zhaolong Li   Group: iGEM19_SDU_CHINA   (2019-10-16)


This part contains YF1, FixJ, PhlF, EsaI, TraR and CcdB.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3869
    Illegal NheI site found at 3892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4808
    Illegal XhoI site found at 3712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 1259
    Illegal NgoMIV site found at 1552
    Illegal NgoMIV site found at 1646
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1427
    Illegal AgeI site found at 3655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1316
    Illegal BsaI site found at 5068
    Illegal BsaI.rc site found at 200
    Illegal SapI.rc site found at 3127


Design Notes

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Source

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References